November 2014

Our main news this month is that Annisa’s paper has been accepted for publication in The Journal of Neuroscience!  Entitled ‘A distinct sub-type of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment’, it not only describes different, functionally distinct classes of dopaminergic cells in the olfactory bulb, but also characterises a novel form of plasticity in one of these classes.  It was a lot of work from Annisa and Elisa, as well as a significant contribution from our project student Rob Chesters, and they can be rightly proud of what they’ve produced.  The manuscript will be Open Access as soon as it’s out, and we’ll post the appropriate link here next time so you can read it for yourselves.

Adna and Matt thinking very hard about the AIS

Adna and Matt thinking very hard about the AIS

Elisa presenting her poster at SfN 2014

Elisa presenting her poster at SfN 2014

Annisa's AIS is this big

Annisa’s AIS is this big

Annisa found out about the paper when she was still in Washington DC, where the lab attended this year’s Society for Neuroscience Annual Meeting.  Adna, Annisa and Elisa all presented posters and all were very well received, despite us book-ending the meeting with Saturday and Wednesday afternoon timeslots (we’ll get luckier next time, because we’re overdue on that now…).

Overall the meeting was great, with a nice buzz around the meeting halls, some impressive plenaries, and a lot of really useful contacts made. Plus everyone apart from Matt made the most of being in the US afterwards: Elisa to go and visit her collaborators at Harvard, Adna to scope out potential post-docs in New York, and Annisa went to see the Space Shuttle Discovery.

While we were in DC our newest lab member Darren held the fort admirably, and even generated some quality data!  Darren’s on a joint PhD studentship between our department and the SGDP at the Institute of Psychiatry, and in his rotation with us has already demonstrated a knack for patching that means we hope we can persuade him to stick around…

Finally, we’re continuing to spread our influence as widely as possible.  Matt recently interviewed to become an inaugural member of the FENS-Kavli Network of Excellence, and spent a fantastic day advising the Theatre-Rites production company on its plans for building a human brain to stick in a puppet. And Elisa was interviewed and accepted as a Brilliant Club mentor, which means she’ll be teaching neuroscience to widen access to university-level education for outstanding pupils from non-selective state schools.

Tired poster presenters in the Wednesday afternoon "graveyard slot"

Tired poster presenters in the Wednesday afternoon “graveyard slot”

This is Gao, new honorary member of the Grubb Lab.

This is Gao, new honorary member of the Grubb Lab.

Post-poster reward of chili cheese fries and half smokes at Ben's Chili Bowl

Post-poster reward of chili cheese fries and half smokes at Ben’s Chili Bowl

September 2014

It’s been a full-on, productive summer here. We’ve written and submitted not one, but two important papers from the lab! Annisa’s first-author story came back from The Journal of Neuroscience with promising reviews, so we’re revising that for re-submission very soon. Mark and Adna’s joint first-author story has just been submitted to Neuron, and we’re all keeping everything crossed for a similar reception there too… Add Matt’s Wellcome Trust Senior Fellowship application to the mix and it’s been a big few months for GrubbLab writing!

Away from the word processor, Adna went to Transylvania and built a 2-photon microscope, then came back and has been going crazy for AIS live-imaging. Annisa presented her recent work at FENS in Milan, and has just disappeared to hunt for post-docs and quality beer in Berlin. And Elisa, as well as getting our slice rig up and functional, won herself an iPad for being an outstanding young behavioural neuroscientist, and got selected by King’s as a post-doc representative on a ‘Life Beyond the PhD’ workshop in the Queen’s backyard (Windsor).

We were all in one place long enough, though, to hold the annual charred food fest that is the (in)famous GrubbLab BBQ, and if you don’t believe us, here’s the evidence!

BBQ_2

Annual Grubb Lab BBQ

June 2014

The sad news this month is that Grubb Lab inaugural member Mark has left us, and the country, to go and cure diseases in California. He’ll be sorely missed, but his leaving party was great fun and we’ll make sure we all stay in touch – not least because we’ve got some exciting papers to write!

Better news is that Elisa has officially started her Sir Henry Wellcome Fellowship. We also found out she was awarded a highly competitive Human Frontier Science Program Fellowship too, but she had to decline that one because unfortunately they don’t let you hold two of these things at once… We made a start to her international networking by visiting the Lledo Lab in Paris in February to chat all things olfactory bulb, and came away with some great ideas for experiments. Talking of which, both Annisa and Elisa have been pushing the boat out collecting the final (hopefully!) data for our first olfactory bulb paper, so it’s been productive recently!

In the meantime, Adna’s been battling valiantly with live AIS imaging, and has continued her jet-setting lifestyle, presenting a poster at the Brain Conference on Controlling Neurons, Circuits and Behaviour in Copenhagen in April, and winning a place on the hugely prestigious Transylvanian Experimental Neuroscience Summer School.

And finally, Matt’s become a dad! Still struggling with extreme sleep deprivation and a world that’s suddenly been turned on its head, but absolutely loving it.

January 2014

DeadSea

Matt in the Dead Sea

Long time no news, but that doesn’t mean we haven’t been busy…

Mark’s now a doctor (insert applause here), having successfully defended his thesis just before Christmas.  He also managed to present his latest project at SfN in San Diego in November, and to find himself an exciting new post-doc position in the Mucke Lab at the Gladstone in San Francisco.  But we’re not letting him leave just yet – he’s in the middle of a mini post-doc here that’ll hopefully polish off a story that we’ll look to publish as soon as we can.

In fact, Grubb Lab alumni have a pretty good track record at finding themselves positions once they’ve left us.  As well as Mark’s new post-doc job, Shivali – our MSc project student from the summer – has now started a PhD in Nottingham, while Tom W, who did a series of different projects with us a few years ago, has got himself on the highly competitive MD-PhD programme at UCL.  We’re also waiting for news from Rob, another undergraduate project student from a couple of years ago, who’s currently in the process of applying for PhDs and may even end up back in this department.

Since the summer we’ve had to say goodbye to Dutch Dennis, who got us up to scratch with our image analysis and who broke all records for Holland Masters grades (apparently).  We’ve been trying to get him back for a PhD but he’s stubbornly staying in Amsterdam for the moment.  And we had another highly successful MRes project student from the 4-year Centre PhD course – Sam learnt how to patch cultured dentate granule cells and got himself and us some publishable data within 3 months, but he’s now off sampling what the rest of our department has to offer.

The good news is that we have a new permanent member of the lab!  Elisa Galliano has just joined us as a post-doc, having got her hard-won PhD from the Erasmus Medical Centre in Rotterdam under the supervision of Chris de Zeeuw.  We seemed to spend most of 2013 begging for money to bring Elisa over, but after applying for 7 different funding schemes she managed to go and get herself the very best one possible: a Sir Henry Wellcome Post-doctoral Fellowship from the Wellcome Trust!  Elisa will now be based here at King’s for the next 4 years, but will also be jet-setting to Paris, Harvard and (ahem) Oxford to do some really exciting experiments with her international band of superstar collaborators.  She’s certainly been busy so far, getting acquainted with the beauty of the olfactory bulb and putting together our new baby: the lab’s new slice patch rig.

In the meantime, Adna successfully upgraded to full PhD student status, went off to Crete for an ISN workshop where she learnt a great deal about German gardening, and presented a poster at the Cambridge Neuroscience Symposium celebrating the 50-year anniversary of the Hodgkin-Huxley Nobel Prize.  Annisa’s been working hard on a project that should see the light of day very soon now, as well as being responsible for the grand new-look website, and Matt went off to a great little interactive Axons in the Desert meeting in Israel and managed to fit in a hugely enjoyable visit to the Mizrahi lab in Jerusalem at the same time.

Last but by no means least, the Grubb Lab officially rocked the MRC Centre Christmas Party this year, teaching everyone a thing or two about fashion, style, moves and scrimping by making a physical and metaphorical visit to the Thrift Shop.

Happy New Year from all of us, and here’s to a great 2014!

 

June 2013

Mark emerging from the darkness

Mark emerging from the darkness

We’ve been out and about recently, taking our science to the Great British public.  At the Science Museum, Matt volunteered on our Centre’s stall in the recent Life Game exhibition, explaining how messing around with neuronal activity might help us tackle serious diseases.  Then there was the MRC Centre Science Spectacular evening, held in conjunction with the ‘Photo51: From DNA to the Brain’ exhibition at Somerset House.  Here, Matt & Mark ran a surprisingly successful live demo of optogenetic activation plus calcium imaging, and you can see a lovely clear picture of Mark in front of our giant results screen here!

Mark and Adna also presented at the recent Tri-Divisional PhD Day, with Adna being highly commended on her poster and Mark scooping joint first prize for his excellent 3rd-year talk.  Well done guys!

And finally we have another new recruit to the lab: Dennis Kruijssen, who’s joined us for a few months on an internship from his MSc course at the Vrije University in Amsterdam.  He’s already got properly stuck in to his experiments, and has introduced us to some truly disgusting licorice, so it’s ups and downs so far…

April 2013

Annisa, Adna and Mark at BNA 2013, Barbican, London.

Annisa, Adna and Mark at BNA 2013, Barbican, London.

Mark’s paper is now out in J Neurosci and thanks to Wellcome’s Open Access policy it’s available for all to read here and here – enjoy!

Braving the hottest, most cramped and most inaccessible poster board layout known to science, Adna, Annisa and Mark all presented at the recent BNA Festival of Neuroscience just up the road at the Barbican.  Some good feedback & discussion, and some interesting sessions at the conference too, so we’re hoping the BNA sticks to London in the future.

More team member changes, as usual – Tom finished his rotation project with us, and we now have an MSc student for the summer, Shivali Kohli, who’s looking at optogenetic activation of calcineurin-dependent transcription factors.  And we’re hoping that Elisa Galliano will join us for a post-doc soon.  Matt went over to her current lab in Rotterdam to give a talk in March, and to work on possible projects and funding for her to study olfactory bulb plasticity here.  The applications are underway, so fingers crossed!

Lastly, in non-science lab news, Matt got married on April 13th!  A fantastic day despite the British weather, and life as an honest man is agreeing with him very well so far.

February 2013

Apologies for the long time since the previous post, but we have been busy!  The best and most important development since then is news of our first laboratory publication: Mark’s first author paper on calcineurin signalling and AIS plasticity features no less than six current and past Grubb Lab members, and is currently in press at the Journal of Neuroscience.  Congratulations everyone!

We also just found out that the lab was successful in bidding for a Research Grant from the Royal Society.  This will add UV-based calcium imaging to the already impressive array of things our confocal can do for us, so we’re itching to start playing with the new equipment as soon as possible.

Not too much travelling at the moment while we gear up for bigger things in 2013, but we had a very local zebrafish imaging meeting in December where Adna got to present her initial data, and Matt went to two different Wellcome fundee meetings in the space of two weeks recently where it was great to interact with scientists across all sorts of biological disciplines.

Personnel-wise, we have a new MRes roton Tom Ryan in the lab, who’s getting involved with olfactory bulb development and plasticity in vivo and producing some lovely 3D cell images.

Away from the bench, we had a Watkinswine-fuelled celebratory dinner to mark our 36-month anniversary as a lab (why not?), a Spiced-up Christmas party season, and we even scored free tickets to go and look down on the department from the top of the Shard!

August 2012

Olympic fever is finally dying down in London and things are getting back to something resembling normality in the lab after a busy old summer.

Rosie finished off her MRes project with us and she’s gone on to a PhD in the Keck lab.  There she’ll continue her interest in all things plastic, and hopefully add some extra data to the interesting results she already produced with us.  And as part of the reciprocal GrubbLab-KeckLab exchange programme we’ve now got Adna back after her spiny summer sabattical.  She’s starting a PhD in collaboration with Martin Meyer’s lab, looking at AISs in zebrafish – a really exciting new direction for us.

We’ve also had two week-long work experience students learning about neuroscience in London; what’s more, both Eleanor and Grace managed to produce useful data in their short time here.  Not the most accurate reflection of normal scientific progress, but at least we haven’t put them off for good…

Finally, the traditional Grubb Lab summer party went non-traditionally barbeque-free – we ate loads of chilli and delicious guacamole on Matt’s temporary riverside balcony instead.  Oh, and believe it or not we’re still waiting on Mark’s sausages.

April 2012

Transition time in the lab right now.  Adna, Rob and Tom all did a grand job of polishing off their respective projects this month, having all produced some really useful data.  Thanks guys!  But we’re not quite down to a skeleton crew because Rosie just joined us on an MRes rotation on synaptic scaling.  She’s got mini recordings already, so things are looking good…

Of the more permanent lab members, Matt went to Goettingen recently to visit Andreas Neef’s lab, giving a talk, getting his hands dirty in the lab and talking a lot of extremely interesting theoretically-biased action potential biology.  Hopefully there might just be some fruitful collaborations there in the future.

Back in London, Annisa’s been working hard at taking her in vitro findings in vivo, while Mark’s still grappling with signalling pathways.  The biggest news, though, is the sausage-making kit we got Mark for his birthday (pictures to follow soon!) – we’re expecting gourmet butties very very soon…

January 2012

It’s taken us a while to warm up this year, but the lab’s now properly back in business with data flooding in all over the place.  This is not least because of two new arrivals: Adna’s patching like a professional already, and Rob’s getting thoroughly into his AIS analysis (see Students page for more details).  And because of Matt’s shameful absence from the otherwise stellar Grubb lab showing at the departmental Christmas FancyDressFest, we treated ourselves to a team non-Christmas dinner this month – you can ask Tom whose curry was nicest because he finished all of them off…